Fig. 4: Fluorescence of MB in the presence of varying concentrations of R9 upon addition of target strand. | Communications Chemistry

Fig. 4: Fluorescence of MB in the presence of varying concentrations of R9 upon addition of target strand.

From: Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors

Fig. 4

A Fluorescence of MB in HP confirmation with varying amounts of R9 peptide in solution that cover above (≥250 μM-green line) and below (≤125 μM-blue line) the concentration required for coacervate formation. This can be observed by the large drop in detected fluorescence in the lower concentrtions. Uncertainties are the results of the sample deviation from three repeats. B Time trace of Cy5/Cy3 fluorescence ratio upon addition of 300 nM target strand in varying concentrations of R9 peptide (ATP constant at 800 μM). C Time trace of the relative Cy5/Cy3 ratio (i.e., signal) change of a 1:1 MB:target reaction in buffer or coacervate (normal concentrations) conditions.

Back to article page