Fig. 5: Following biosensing on a microscope. | Communications Chemistry

Fig. 5: Following biosensing on a microscope.

From: Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors

Fig. 5

A Confocal fluorescence microscopy image of R9-ATP coacervates with HP form MB sequestered into the coacervates. Excitation 520 nm, detection 540–740 nm. B Fluorescence spectra obtained from confocal fluorescence microscopy of coacervates seen in A at different times upon addition of a 1:2 ratio of MB:target. Spectra are obtained by averaging over the entire field of view. C Time trace of Cy5/Cy3 fluorescence ratio of confocal image seen in A.

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