Table 2 Fluorescence quantum yield of the dyes on the MB in varying confirmations (HP and dsDNA) and conditions (buffer and coacervate)

From: Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors

 

Buffer

Coacervate

 

HP

dsDNA

HP

dsDNA

Cy3 donor

0.199 ± 0.01

0.16 ± 0.01

0.52 ± 0.04

0.62 ± 0.04

Cy5 acceptor

0.20 ± 0.01

0.28 ± 0.01

0.39 ± 0.03

0.36 ± 0.02

  1. Determinations based on secondary standards (rhodamine B and oxazine 720)30, uncertainties are based on propagating uncertainties from standards and experimental spectroscopy.
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