Fig. 2: Screening of an in-house collection of 63 KRED wild-type enzymes toward 1a reduction. | Communications Chemistry

Fig. 2: Screening of an in-house collection of 63 KRED wild-type enzymes toward 1a reduction.

From: Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor

Fig. 2

Ketone 1a (c = 3 g l−1 equivalent to 10 mm) was reduced in the presence of a glucose/glucose dehydrogenase system for NAD(P)H regeneration. KREDs are ordered based on their phylogenetic relationship (iTOL v5, EMBL)65. Concentrations of target product 2a and its diastereomer 2b ((R,S)-cis alcohol) are indicated. KRED 54 (Ssal-KRED) yielded the highest conversion and trans-diastereoselectivity within the collection. EV empty vector (negative control). Source data for this figure is available (Supplementary Data 2).

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