Fig. 5: Mutations and fold-improvement of Ssal-KRED hit variants.
From: Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor
Ssal-KRED hit variants were obtained via hot spot engineering (HSE), machine learning (ML) and iterative site mutagenesis (ISM). Positions are color-shadowed in accordance with the library they were investigated in (for reference, see Fig. 3a, c). The fold-improvement over the wild type (FIOWT) was calculated from fold-improvement over the parent (FIOP) values. AA amino acid, − not applicable.
