Fig. 6: Residues targeted in the iterative site mutagenesis approach.
From: Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor
Homology model of M3 (F97W_A238K_L241M_M242W_Q245S_L316M_T342M) with bound 1a (blue) and cofactor (yellow). Catalytic residues are depicted in purple. Mutated residues in the active site and tunnel entrance are displayed in red, surface mutations L316M and T342M are not shown. Positions in the substrate or cofactor environments targeted for the iterative site mutagenesis libraries are colored in accordance with the libraries they are located in: 2-site CSM library L11 (orange), SSM libraries L12 (marine blue) and 2-site CSM library L13 (green).
