Table 1 Comparison of KRED-mediated syntheses of 2a

From: Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor

Parameter

Ssal-KRED (WT)

Ssal-KRED_M6

s/ea

5

5

50

KREDb

20 mg

2 g

0.2 g

CRSc

iPrOH

iPrOH

Glc/GDH

Substrate 1a

100 mg

10 g

10 g

Conversiond

26%e

99%

>99%

de (trans)

>99.5%

99.7%

99.9%

HPLC-purityf

n.d.

99.7%

99.5%

Reaction time

24 h

30 h

27 h

Temperature

23 °C

23 °C

23 °C

  1. n.d. not determined, WT wild-type enzyme.
  2. aSubstrate-to-enzyme ratio.
  3. bAs lyophilized enzyme lysate.
  4. cCofactor recycling system.
  5. dDetermined by achiral HPLC.
  6. eReaction stalled.
  7. fDetermined by chiral HPLC.
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