Table 2 Kinetic characterization of selected Ssal-KRED variants using ketone 1a for the synthesis of 2a

From: Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor

Ssal-KRED variant

app. KM, [µM]

app. kcat, [min−1]

app. kcat/KM, [min−1 mM−1]

rel. kcat

WT

11.9 ± 1.1

0.49 ± 0.02

41

1

M1

162.8 ± 7.8

5.8 ± 0.1

35.5

12

M2

175.2 ± 9.9

6.2 ± 0.2

35.6

13

M3

299.7 ± 7.9

15.3 ± 0.4

51.2

31

M4a

1668 ± 103

45.8 ± 2.4

27.5

94

M5

471.7 ± 34.6

21.3 ± 1.0

45.2

43

M6

143.5 ± 5.5

31.5 ± 0.8

219.7

64

  1. Source data for this table is available (Supplementary Data 6).
  2. WT wild-type enzyme.
  3. aSubstrate saturation was not reached.
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