Fig. 2: Preliminary screening for diastereotope selective amination of the ketone 2a-d with immobilized whole-cell forms of three (R)-selective and three (S)-selective transaminases in batch mode. | Communications Chemistry

Fig. 2: Preliminary screening for diastereotope selective amination of the ketone 2a-d with immobilized whole-cell forms of three (R)-selective and three (S)-selective transaminases in batch mode.

From: Transaminase-catalysis to produce trans-4-substituted cyclohexane-1-amines including a key intermediate towards cariprazine

Fig. 2

Reaction conditions: 2a-d (5 mM), (R)-3 (15 mM, 3 eq., for ArR-TA, ArR-TAmut, AtR-TA), (S)-3 (15 mM, 3 eq., for ArS-TA, VfS-TA, CvS-TAW60C), PLP (0.05 mM, 5 mol%) and immobilized whole-cell transaminase (imm-whc-TA, 50 mg) in sodium phosphate buffer (100 mM, pH 7.5) shaking at 600 rpm for 24 h at 30 °C. Conversion (left scale of the Y-axis) and diastereomeric excess (de: right scale of the Y-axis) were determined by GC.

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