Fig. 4: Engineering wetting between coacervates formed with diverse palindromic sequences at 37 °C.

The coacervates were prepared using a mixture containing a final concentration of 0.06 µM of A₁₅₀₀ and 1.5 µM of the respective B_x. a Illustration of three distinct coacervate structures (C₁ in magenta, C₂ in green, and C₃ in cyan) synthesized from different binders (Atto_647N–B_8-1, Atto₄₈₈–B_8-2, and Atto₅₆₅–B_8-3) with various palindromic sequences (P_8-1, P_8-2, and P_8-3, as showed in Fig. 2b, c). The experiments were performed at 37 °C with gentle rotation at 80 rpm unless otherwise specified. b Wetting behavior between distinct C₁ and C₂, leading to the formation of mixed coacervates. c Wetting behavior between C₁ and C₃, resulting in the formation of core–shell structures. d Wetting behavior between C₂ and C₃, contributing to the formation of core–shell structures. The experiments were carried out at 37 °C without rotation to minimize collisions. e Behavior during RNase H-initiated, slow coacervation of C₁ in a dispersion of preformed C₂. 0.1 U µL⁻¹ RNase H and preformed C₂ were introduced into the complex comprising 0.06 µM A₁₅₀₀, 1.5 µM of B_8-1, and 2.25 µM RNA invader. f Behavior during RNase H-initiated, slow coacervation of C₁ to preformed C₃. 0.1 U µL⁻¹ RNase H and preformed C₃ were introduced into the complex comprising 0.06 µM A₁₅₀₀, 1.5 µM of B_8-1, and 2.25 µM RNA invader. The experiments were conducted at 37 °C with gentle rotation at 80 rpm. Scale bars: 10 μm.