Fig. 2: ATP/AzoDiGua aggregates are fluorescent upon assembly of non-fluorescent constituents.

A Fluorescence emission spectra (Exc: 400 nm) of ATP alone (1 mM), AzoDiGua alone (1 mM), and the resulting mixture after 10 min of assembly. B Fluorescence microscopy image (Exc: 475/20 nm; Em: 500–800 nm) of the supramolecular ATP/AzoDiGua aggregates shown in Fig. 1Cright. The scale bar is 50 µm. All experiments are done at room temperature in 50 mM Tris HCl buffer solutions (pH = 7.4).