Fig. 5: Enzymatic control of the ATP/AzoDiGua assembly and fluorescence.

A Photographs of ATP/AzoDiGua suspension before (left) and after addition of alkaline phosphatase (ALP, 0.025 µg/µL) for 10 min (right). B Fluorescence intensity (Exc: 400 nm; Em: 650 nm) as a function of time for the ATP/AzoDiGua suspension without (blue symbols) or with (red symbols) added ALP to ATP/AzoDiGua suspension at t = 0, or for AzoDiGua alone (1 mM, green symbols) with added ALP at t = 0. For all experiments, the initial ATP/AzoDiGua suspension results from the self-assembly of ATP (1 mM) and AzoDiGua (1 mM) for 10 min. All experiments are done at room temperature in 50 mM Tris HCl buffer solutions (pH = 7.4).