Fig. 2: Direct glycosylation analysis from intact SiLu™Lite MSQC4 mAb expressed in CHO cells. | Communications Chemistry

Fig. 2: Direct glycosylation analysis from intact SiLu™Lite MSQC4 mAb expressed in CHO cells.

From: Direct glycosylation analysis of intact monoclonal antibodies combining ESI MS of glycoforms and MALDI-in source decay MS of glycan fragments

Fig. 2

a Direct infusion ESI FT-ICR mass spectrum of SiLu™Lite MSQC4. b Maximum entropy deconvolved ESI FT-ICR mass spectrum of SiLu™Lite MSQC4. Five major mAb glycoforms were detected. Some minor species were associated with phosphate adducts. c MALDI-ISD FT-ICR mass spectrum of SiLu™Lite MSQC4. Solely glycan fragments are indicated (see Supplementary Notes: Evaluation of protein fragment ions and matrix adducts). d Glycosylation profiles obtained by MALDI-ISD MS considering 0,2A fragments and two glycopeptide analyses by LC-MS (see Fig. S7).18 ID = Inconsistently detected. Glycan residue symbols: blue square, N-acetylglucosamine; green circle, mannose; yellow circle, galactose; red triangle, fucose.

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