Fig. 8: LC-MS analysis of tyrosine modification on TDP1. | Communications Chemistry

Fig. 8: LC-MS analysis of tyrosine modification on TDP1.

From: Targeted sulfur(VI) fluoride exchange-mediated covalent modification of a tyrosine residue in the catalytic pocket of tyrosyl-DNA phosphodiesterase 1

Fig. 8

a ReSpect deconvolution of the MS1 scans from TDP1(148-608) incubated with DMSO and the compounds 2e, 3b, 3c, 3f, and 3g at a ratio of 1:65 (enzyme/compound) (the significant species shown in red). b A representative MS/MS spectrum of covalently modified Y204 with 3c through the displacement of fluorine. c The normalized %modification of 3c on tyrosine residues of TDP1 calculated from the sum of grouped abundances of the peptides. Y204 harbored the highest grouped abundances based on Label-Free Quantitation (LFQ) values and was used to normalize the %modification.

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