Table 1 Strains and plasmids used in this study
Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
Escherichia coli | ||
E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(rK− mK+), e14− (mcrA−), supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB+, lac Iq, lacZΔM15] | Takara Bio Inc. |
E. coli BL21-AI | F–, ompT, hsdSB (rB– mB–), gal, dcm, araB::T7RNAP-tetA | Thermo Fisher Scientific |
Bacillus subtilis | ||
B. subtilis 168 | trpC2 | Lab stock |
B. subtilis ATCC6633 | Wild-type (NBRC 3134) | NITE Biological Resource Center |
B. subtilis RB14 | Wild-type | |
KB04 | ΔppsABCDE::fenF-mycA-mycB-mycC-kan, sfp::lpa-14-cat | |
BL043 | ΔppsABCDE::erm-PrplGB-fenF-mycA-mycB-mycC-kan, sfp::lpa-14-cat | This work |
BL063 | BL043 ΔsigF | This work |
BL082 | BL063 ΔmycAL-mycACP::pheS*-spec | This work |
BL123 | BL063 ΔmycAL::ituAL | This work |
BL134 | BL063 ΔmycACP::ituACP | This work |
BL135 | BL063 ΔmycAL-mycACP::ituAL-ituACP | This work |
BL136 | BL063 ΔmycAL::chimaeric AL domain 1 (region 1 derived from mycAL and regions 2, 3, 4, and 5 derived from ituAL) | This work |
BL137 | BL063 ΔmycAL::chimaeric AL domain 2 (regions 1 and 2 derived from mycAL and regions 3, 4, and 5 derived from ituAL) | This work |
BL138 | BL063 ΔmycAL::chimaeric AL domain 3 (regions 1, 2, and 3 derived from mycAL and regions 4 and 5 derived from ituAL) | This work |
BL139 | BL063 ΔmycAL::chimaeric AL domain 4 (regions 1, 2, 3, and 4 derived from mycAL and region 5 derived from ituAL) | This work |
BL083 | BL063 Δerm-PrplGB-fenF-mycAL-mycACP::spec | This work |
XyyyZ | BL063 ΔmycAL::ituAL_XyyyZ | This work |
BL113 | ΔppsABCDE::spec, sfp::lpa-14-cat, ΔsigF | This work |
BL144 | ΔppsABCDE::spec-ituA’-ituB-ituC, sfp::lpa-14-cat, ΔsigF, srfAB::kan | This work |
BL127 | ΔppsABCDE::erm-PrplGB-ituD-ituA-ituB-ituC, sfp::lpa-14-cat, ΔsigF, srfAB::kan | This work |
BL128 | BL127 ΔituAL::mycAL | This work |
F208L | BL127 ΔituAL::ituAL_F208L | This work |
K287R | BL127 ΔituAL::ituAL_K287R | This work |
A332T | BL127 ΔituAL::ituAL_A332T | This work |
F208L, K287R | BL127 ΔituAL::ituAL_F208L, K287R | This work |
F208L, K287R, A332T | BL127 ΔituAL::ituAL_F208L, K287R, A332T | This work |
F208X | BL127 ΔituAL::ituAL_F208X | This work |
MycAL_L208X | BL127 ΔituAL::mycAL_L208X | This work |
Fungi | ||
Rhizoctonia solani | Wild-type (MAFF 242303) | Genebank Project, NARO |
Yeast | ||
Saccharomyces cerevisiae BY4742 | MATalpha his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | Horizon Discovery, Ltd. |
Plasmids | ||
pUC19 | Cloning vector | New England Biolabs |
pUC19-mycU-erm-PrplGB-fenF-ituAL-mycACP-mycAD | QuikChange template plasmid for generating XyyyZ strains | This work |
pUC19-mycU-erm-PrplGB-ituD-ituAL-ituACP-ituAD | Construction of BL127 QuikChange template plasmid for generating F208X strains | This work |
pUC19-mycU-erm-PrplGB-ituD-mycAL-ituACP-ituAD | Construction of BL128 QuikChange template plasmid for generating MycAL_L208X strains | This work |
pET28a | Cloning vector for protein expression | Merck |
pET28a-ituAL-ACP1 | Expression vector for His-tagged ItuAL_WT-MycACP | This work |
pET28a-ituAL_F208G-ACP1 | Expression vector for His-tagged ItuAL_F208G-MycACP | This work |
