Fig. 8: Tetrazine-triggered ADC cleavage in vitro.

a Structure of the diabody-based anti-TAG-72 ADC used in this work12; b MMAE release from ADC in reaction with ortho-substituted tetrazines in 50% mouse plasma at 37 °C after 24 h (mean ± SD, n = 3) and; c time-dependent MMAE release from ADC in the presence of selected tetrazines in 50% mouse plasma at 37 °C up to 24 h.