Fig. 5: Summary of RdRp assay. | Communications Chemistry

Fig. 5: Summary of RdRp assay.

From: Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins

Fig. 5

A General scheme of RdRp polymerase assay. Hairpin RNA substrate with template-product scaffold. B Gel-based JEV RdRp assay analyzed by western blot and SDS-PAGE (lane C) positive control – reaction with natural NTPs; (lane C*) reaction with CESQTP, GTP, ATP, UTP. For the uncropped gels see Supplementary Fig. 1. C Proteinase K or Trypsin digestion of the RNA-protein conjugate; (lane 1) template; (lane 2) negative control – reaction without CTP; (lanes 3 and 5) positive control – reaction with natural NTPs; (lanes 4 and 6) reaction with CESQTP, GTP, ATP, UTP. D RNA digestion for nano LC-MS/MS analysis and spectrum of 46_RNA_HPC4_JEVNS5 with identification of K269 (m/z acquired: 586.26 Da; Supplementary Fig. S45).

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