Table 1 Summary of cross-linking reactions between 36_vRNA_1gCESQ and proteins

From: Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins

Protein

Molecular weight [kDa]

RNA binding

No. of Lys residues

Conversion %

BSA

69.3

no

60

0%

T7 RNAP

99

no

66

0%

SSB

18.9

weakly

6

9%

HIV rt

117

yes

108

15%

JEV NS5

103.3

yes

58

43%

YFV NS5

103.3

yes

60

40%

SC_RdRp

62.2

yes

72

traces

SC_NP

45.6

yes

31

traces

  1. Note: Conversions were determined from the gel using ImageJ quantificator.
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