Fig. 3: DNA enrichment in the LIPS droplet. | Communications Chemistry

Fig. 3: DNA enrichment in the LIPS droplet.

From: Metastable phase-separated droplet generation and long-time DNA enrichment by laser-induced Soret effect

Fig. 3

a Schematic illustration of the concept of long-time DNA enrichment in LIPS droplets. b, c Phase-contrast image, fluorescent image of dextran, and fluorescent image of DNA observed by epifluorescence microscopy from left to right. The droplet was induced by laser irradiation for 5 min to the upper PEG-rich phase of DEX 6 wt.% and PEG 2.6 wt.% + λ-DNA (48,502 bp) 29 ng/µL. b t = 0 h. c t = 18 h after the laser is disabled. The droplet was visualised using a DNA intercalator and fluorescently labelled dextran. The DNA was successfully incorporated into the generated dextran-rich droplet. Scale bar = 10 μm.

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