Fig. 1: Enzymatic activities of WT, F393H, and F87A/F393H of P450BM3 with C7ProPhe for oxidation of styrene.

Reaction conditions: P450BM3 (1 μM), C7ProPhe (100 μM), styrene (saturated), NADPH (5 mM), at room temperature for 5 min. The TOF and ee values are presented as mean ± s.d. (n ≥ 3 independent experiments).