Fig. 1: Coevolving residue positions and their role in GAF domain allostery.
From: Coevolving residues distant from the ligand binding site are involved in GAF domain function
Schematic showing an approach to determine coevolving residues and delineating their role in the allosteric regulation of GAF domains. GAF domain sequences were aligned, and statistical coupling analysis (SCA) was performed to detect coevolving residue positions. The scoring matrix obtained from SCA was then used for clustering to determine the cluster of coevolving residues with highest scores, which were then mapped on to a GAF domain structure. MD simulations followed by biophysical assays with biosensors were then used to determine the role of individual coevolving residue positions in the allosteric regulation of GAF domains.
