Fig. 2: SCA reveals a cluster of coevolving residues in GAF domains.
From: Coevolving residues distant from the ligand binding site are involved in GAF domain function
A Color-coded heatmap showing pairwise scores of individual residue positions in GAF domains obtained from SCA. The positions of the nine coevolving residues are indicated. The area in the heatmap where the coevolving residue positions intersect is highlighted by a white box. B Cartoon representation of the GAFa domain of PDE5 (PDB: 2K3122) showing the coevolving cluster of residues (spheres in light blue) in the GAF domains. C Cartoon representation of the GAFa domain of PDE5 showing residues (side chains as sticks in pink) within a 5 Å distance from cGMP (left panel), coevolving residues (spheres in light pink; rest of the coevolving residues in light blue) within 5 Å distance from cGMP (middle panel) and coevolving residues L267 and F295 selected for further functional characterization (dark blue spheres, right panel).
