Table 3 Comparative muropeptide analysis of PG extracted from cells grown in TY or MM

From: A software tool and strategy for peptidoglycomics, the high-resolution analysis of bacterial peptidoglycans via LC-MS/MS

 

TY

  

MM

  

Unpaired t test

 

Average SD

Average SD

P value (significance)

Monomers (inc. porins)

30.72%±1.06%

35.57%±1.17%

 

Dimers (inc. porins)

49.65%±0.85%

42.94%±0.31%

 

Trimers (inc. porins)

19.63%±0.22%

21.49%±0.90%

 

3–3

62.56%±0.45%

64.68%±0.31%

0.003 (**)

4–3

37.44%±0.45%

35.32%±0.30%

0.002 (**)

Cross-linking index

31.30%±0.49%

28.53%±0.43%

0.002 (**)

AEJX monomers (excl. AEJA)

4.86%±0.21%

1.56%±0.34%

 

AEJX dimers (excl. AEJA)

16.01%±0.50%

0.50%±0.06%

 

AEJX trimers (excl. AEJA)

5.70%±0.05%

0.12%±0.01%

 

All AEJX

26.58%±0.52%

2.18%±0.41%

<0.001 (***)

AEJAX monomers (excl. AEJAA)

4.01%±0.09%

3.20%±0.17%

 

AEJAX dimers (excl. AEJAA)

5.28%±0.11%

1.78%±0.27%

 

AEJAX trimers (excl. AEJAA)

7.31%±0.26%

0.67%±0.14%

 

All AEJAX

16.59%±0.29%

5.65%±0.41%

<0.001 (***)

Anh monomers (inc. porins)

2.18%±0.08%

2.26%±0.15%

 

Anh dimers (inc. porins)

5.95%±0.17%

4.98%±0.16%

 

Anh trimers (inc. porins)

0.89%±0.02%

0.07%±0.02%

 

All anhydro

9.02%±0.14%

7.77%±0.32%

 

Chain length

18.37±0.23

21.08±1.03

0.011 (*)

Porin monomers

3.83%±0.63%

5.28%±1.26%

 

Porin dimers

1.17%±0.23%

3.08%±0.60%

 

Porin trimers

0.23%±0.04%

0.75%±0.17%

 

All porins

5.23%±0.87%

9.12%±2.01%

0.037 (*)

Back to article page