Fig. 2: Screening optimization of STAAR lipids. | Communications Chemistry

Fig. 2: Screening optimization of STAAR lipids.

From: Multicomponent thiolactone-based ionizable lipid screening platform for efficient and tunable mRNA delivery to the lungs

Fig. 2

a Amines (Ai), Bj derivatives and acrylates (Ck) used for lipid screening. b Scheme summarizing the strategy followed for the two screening phases. c Total flux (entire mouse body) results of screening phase 1, where B was optimized by combining different acrylates and Bj derivatives while keeping polar head (A1) fixed. Mice were intramuscularly injected (i.m.) with mRNA-Luc-loaded LNPs at an mRNA dose of 0.05 mg/kg. Luminescence imaging acquisition was performed at 4 h post-treatment and total flux was quantified. In vivo mLuc expression displayed in a heat map (n = 3 biologically independent samples). Data are presented as mean values. d Total flux results table of Screening phase 2 where A and C were optimized by combining different amines and acrylates while keeping B2 fixed. Mice were i.m injected with mRNA-Luc-loaded LNPs at an mRNA dose of 0.05 mg/kg. Total flux luminescence imaging acquisition of the whole mouse body was performed at 4 h post-treatment and total flux was quantified. In vivo mLuc expression displayed in a heat map (n = 3 biologically independent samples). Data are presented as mean values. Below each table, representative luminescence images of each lipid in mice acquired using the IVIS Lumina XRMS Imaging System are shown.

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