Fig. 5: Results of distinguishing cell types based on the signal intensities of lipid ions. | Communications Chemistry

Fig. 5: Results of distinguishing cell types based on the signal intensities of lipid ions.

From: Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization

Fig. 5

a Comparison of fluorescence images of mCAT and cGCS cells. b Volcano plot showing differences in the signal intensities of lipid ions between cell types. c Score plot obtained via principal component analysis. d–i Bar plot showing the differences in the signal intensities of lipid ions in single cells. j–l Comparison of ion images with high signal intensities in mCAT cells. m–o Comparison of ion images with high signal intensities in mCAT cells. In each ion image, the left side shows mCAT cells, and the right side shows cGCS cells. The m/z values of the ions and the assigned lipid ions are shown in (j–o). p–s Enlarged fluorescence and ion image showing a high signal intensity in mCAT cells. t–w Enlarged fluorescence and ion image showing a high signal intensity in cGCS cells. The enlarged regions are indicated by dashed lines in (j–o). Scale bar: 10 μm.

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