Fig. 2: Direct observation of secondary nucleation in the absence and presence of the apoE isoforms and quantification of elongation properties of secondary fibrils. | Communications Chemistry

Fig. 2: Direct observation of secondary nucleation in the absence and presence of the apoE isoforms and quantification of elongation properties of secondary fibrils.

From: A single fibril study reveals that ApoE inhibits the elongation of Aβ42 fibrils in an isoform-dependent manner

Fig. 2

A representative TIRFM images of the secondary fibrils generated on the primary fibril surface (indicated by yellow arrow) in absence of apoE (top panel), in presence of 150 nM apoE4 (middle), and 150 nM apoE3 (bottom). Scale bar = 2 μm. Panels (B, C) show the time evolution of the integrated fibril mass \(M(t)\) (black) and the total number of new fibrils \({N}_{2}\left(t\right)\) (considered as 2⁰ events) (blue) in absence and presence of 150 nM apoE4, respectively. \({N}_{2}\left(t\right)\) increases proportionately with an increase in \(M(t)\). D, E Single fibril tracking data of the primary (black) and secondary fibrils (red) in absence and presence of the apoE3, respectively. F, G Rate of elongation of the primary and secondary fibrils in absence and presence of apoE3, respectively. Rate of elongation of each fibril is represented as a dot here. In (F, G) the error bars represent mean ± SD, *P < 0.05, **P < 0.01 and ***P < 0.001 (from Student’s t test)).

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