Fig. 2: CD analysis of G4 formation by double-stranded DNA in mitochodnria-mimicking conditions.
From: Predictability of environment-dependent formation of G-quadruplex DNAs in human mitochondria
a CD spectra of the double-stranded sequences of L, D1, D2, D4, mt6363, and CSBII. The spectra were measured in 10 mM Na2HPO4 and 1 mM EDTA buffer (pH 7.0) with the following additions: (i) buffer only, (ii) 150 mM KCl, (iii) 150 mM KCl and 30 wt% 1,3-PDO, (iv) 150 mM KCl and 45% 1,3-PDO, (v) 150 mM KCl and 60 wt% 1,3-PDO, (vi) 150 mM KCl, 30 wt% 1,3-PDO, and 0.5 wt% PEG8000, and (vii) 150 mM KCl, 30 wt% 1,3-PDO, and 1 wt% PEG8000. The CD spectra of the L-s, D1-s, D2-s, D4-s, mt6363-s, and CSBII-s strands in the buffer and 150 mM KCl without crowders were also included. b The relative increase in the molar ellipticity at 265 nm of the double-stranded D2, D4, mt6363, and CSBII sequences. The signals of duplexes in the absence of KCl and cosolutes are defined as 0%, while the signals of single-stranded G-rich strands in the presence of 150 mM KCl are defined as 100%. The experiment was conducted in triplicate, and the standard deviations are represented by the error bars.
