Fig. 2: PG-DA versus PA-DA competitive chemoproteomics experiments.

A, B A LC-MS/MS based proteomics analysis showing enrichment ratio (heavy:light; H:L) of the total proteins identified in this probe versus probe (PG-DA versus PA-DA) competitive chemical proteomics experiment (each probe was used at 500 μM, 6 mins of UV exposure) done with (A) the membrane and soluble proteomes prepared from the mouse brain; and (B) proteomes prepared from different immortalized mammalian cell lines (Neuro2A, BV2 and RAW264.7). Each data point in (A) or (B) represents the mean of the enrichment ratio obtained for the respective protein from two or three biological replicate for a particular proteome, based on the defined filtering criteria for this proteomics experiment. The horizontal dotted line denotes an enrichment ratio ≥ 1.5, and proteins having an enrichment ratio above this threshold were considered specifically enriched by the PG-DA probe, and taken forward for subsequent analysis. Complete details for all the proteins can be found in Supplementary Data 3. Inset in (A), shows high enrichment of the neuronal calcium sensor Hippocalcin (HPCA), and the mitochondrial translocase sub-unit TOMM22 by the PG-DA probe, along with known MAG interactors such as vimentin (VIM), and nucleobindin 1 (NUCB1). (C, D) Categorization of all the proteins specifically enriched by the PG-DA probe based on all experiments described in (A) and (B), using the Panther database categorization into different: (C) Known molecular functions; and (D) biological processes involved in.