Fig. 4: Results of HTRF TR-FRET and X-ray experiments.
From: A bottom-up approach to find lead compounds in expansive chemical spaces
a Summary of the IC50 values obtained by HTRF TR-FRET for the DSF/SPR hits and X-ray screening hits. Error bars correspond to the 95% confidence interval (n = 2) b Examples of the dose-response curves assessed by HTRF TR-FRET, comparing the (+)−JQ1 control (compound 3, black) and three example compounds (blue and orange). Each dot represents the average of two independent replicas, and the error bars correspond to their standard deviation. c X-ray structures of compounds 92 (Supplementary Data 2; PDB 9HT2), 94(Supplementary Data 3; PDB 9HT1), 50 (Supplementary Data 4; PDB 9HT0), bound to BRD4 (BD1). In the picture are illustrated the predicted binding pose (pink) and the resolved crystallographic structure (green).
