Fig. 4: Characterization of enrichment of the in vitro selection experiment by ELONA and HTS.
From: Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection
A Binding studies of three libraries by ELONA assays. Shown is the average and standard deviation of three ELONA replicates. B Evolution of Top50 clusters during the SELEX. This figure presents for the Top50 clusters, the frequency of the whole cluster inside each library (in %). For instance, all the sequences belonging to the cluster 0 are representing 0,0201% of the library of round 6, and 5,2695 % of the library of round 10. Clusters colored in light pink are clusters selected for the binding tests. C Sequences chosen for binding tests based on the NGS analysis. The exact motif present in their sequence is also given. For the sake of clarity, the primer binding regions were omitted. Red, bold-face X indicates the position of the modified nucleotide.
