Fig. 5: Flow cytometry binding analysis of aptamer candidates with PvLDH.
From: Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection
10 nM 5’-FAM-labeled sequences along with a control sequence (Ctrl) were incubated with His-tagged PvLDH (10 µg) in binding buffer at room temperature for 1 hour. After washing and preparation of Ni-NTA agarose beads, the beads were incubated with the aptamer-PvLDH solution for 30 minutes and analyzed by flow cytometry using the Attune NxT Flow Cytometer.
