Fig. 6: Dissociation constant (KD) determination of aptamers.
From: Probing three-dimensional cyclooctatetraene for nucleobase modification in aptamer selection
Various concentrations of 5’-FAM-labeled modified and unmodified aptamers corresponding to sequences A N0 (R2 = 0.98), B N5 (R2 = 0.93), and C N15 (R2 = 0.96) were used to evaluate binding interactions. The binding curves for modified aptamers were analyzed using GraphPad Prism with nonlinear regression (curve fit) based on a one-site total binding model. The calculated KD values and their 95% confidence intervals were used for standard deviation calculations. KD values for the unmodified aptamers were found to be undefined, preventing the calculation of a mean value during the analysis.
