Fig. 4: pH-dependent photoreduction, CTT release, and oxidation of wild-type dCry.
A Illumination of dCry at pH 6.5 resulted in the formation of greater amounts of nsq (left panel), accompanied by lower fractions of asq and inhibition of CTT release (middle and right panels. ▪, the ox state; ●, the asq state; ▲, the nsq state; ▼, the total of the redox states; the hq state was not detected; one representative result is shown. Orange stars, the degree of CTT release represented by the intensity ratio of fragment d to the sum of fragments c and d). The red line represents the fitted curve of asq formation during photoreduction at pH 6.5 (kpr1 ~ 0.06 s−1, Supplementary Table 1). B Illumination of dCry at pH 9.0 led to the formation of nearly pure asq (left panel), and CTT release increased (middle and right panels. The symbols are the same as above). The red line represents the fitted curve of asq formation during photoreduction at pH 9.0 (kpr1 ~ 0.07 s−1, Supplementary Table 1). C pH-dependent nsq fractions of the total semiquinone (asq + nsq) formed during photoreduction. D Relationship between the photoreduction rate (kpr1) and pH. E pH-dependent oxidation rates of asq (kasqox) in dCry after photoreduction.
