Fig. 5: Comparative analysis of S. neptunia Enr13T wild type and ∆nasY1-3 knock-out mutants.

A Molecular network of stieleriacine-related molecular ion features, showing in addition to stieleriacine D and E, six additional mass features of stieleriacine derivatives. Structures of stieleriacine F, H, and I were predicted based on MS/MS fragmentation analysis. Extracted ion chromatograms of B stieleriacine D (m/z 458.326 [M + H]+) and C stieleriacine E (m/z 460.342 [M + H]+) of S. neptunia Enr13T wild type (WT; black), ∆nasY1 (orange), ∆nasY2 (blue) and ∆nasY3 (green).