Table 1 aThermodynamic parameters of anion binding to ScNreA from 10–25 °C

From: Probing the anion binding promiscuity of the soluble nitrate sensor NreA from Staphylococcus carnosus

Anion

Temperature (°C)

Kd (μM)

ΔH (kJ/mol)

TΔS (kJ/mol)

ΔG (kJ/mol)

ΔCp (J/mol K)

Nitrate

25

4.0 ± 0.1

−32.1 ± 0.1

−1.4 ± 0.1

−30.8 ± 0.0

−308.2 ± 21.6

20

2.7 ± 0.1

−30.1 ± 0.1

1.3 ± 0.1

−31.3 ± 0.1

15

2.5 ± 0.2

−28.4 ± 0.1

2.6 ± 0.1

−31.0 ± 0.2

10

1.4 ± 0.1

−27.0 ± 0.1

4.8 ± 0.4

−31.7 ± 0.3

Iodide

25

44.5 ± 2.0

−21.5 ± 0.3

3.3 ± 0.1

−24.9 ± 0.1

242.3 ± 34.9

20

37.7 ± 0.8

−22.5 ± 0.0

2.4 ± 0.1

−24.9 ± 0.1

15

34.7 ± 0.0

−23.5 ± 0.3

1.1 ± 0.3

−24.6 ± 0.0

10

26.6 ± 3.7

−24.7 ± 0.4

0.2 ± 0.0

−24.9 ± 0.4

Nitrite

25

109 ± 11

−17.8 ± 0.0

4.8 ± 0.3

−22.6 ± 0.4

33.1 ± 11.2

20

97.5 ± 10.6

−18.0 ± 0.1

4.6 ± 0.2

−22.5 ± 0.3

15

85.3 ± 5.9

−18.1 ± 0.1

4.4 ± 0.1

−22.5 ± 0.2

10

71.8 ± 6.7

−18.3 ± 0.3

4.1 ± 0.2

−22.5 ± 0.1

  1. aThe best-fit values for each parameter were obtained through a global analysis of three technical measurements for two biological replicates and are reported as the average with standard deviation. The protein and anion samples were prepared in 50 mM HEPES buffer with 50 mM sodium chloride at pH 7.8.
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