Table 2 aKinetic parameters of anion binding to ScNreA at 10 °C

From: Probing the anion binding promiscuity of the soluble nitrate sensor NreA from Staphylococcus carnosus

Anion

kon (× 105) (M−1 s−1)

koff (s−1)

Nitrate

2.70 ± 0.01

3.26 ± 0.49

Iodide

2.45 ± 0.01

12.32 ± 1.06

Nitrite

1.82 ± 0.01

21.39 ± 2.26

  1. aThe kinetic parameters from three technical measurements for two biological replicates are reported as the average with propagated standard deviation. The protein and anion samples were prepared in 50 mM HEPES buffer with 50 mM sodium chloride at pH 7.8.
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