Fig. 2: Cross-platform comparison of protein quantification and detection metrics.

a UpSet plot showing set size and intersection of identified proteins by each platform. 259 shared proteins are quantifiable across all platforms, excluding NULISA. Inclusion of NULISA reduces the number of quantifiable proteins to 36. b Technical and total CVs shown for each analyte on each platform. Technical CVs were calculated using set of technical replicates. Total CVs were calculated using all 78 subjects. c Percentage of analytes that were detected in each number of samples. Each dataset was filtered based on 2/3rds data completeness from each age group. d The detected proteins present in the HPA dataset were binned according to the estimated concentrations from the HPA dataset. The percentage make-up of proteins belonging to these bins are plotted. e The number of FDA approved protein biomarkers identified by each platform.