Fig. 1: Schematic of the rapid glycosylation screening method.

This method is suitable for the rapid characterization of N-glycosylation across multiple glycoprotein production steps (upper left section). By collecting the products and simultaneously preparing internal standards for high-throughput sample analysis (upper right section), coupled with the high-speed analysis capability of MALDI-TOF-MS (lower right section), accurate glycan content information is obtained (lower left section). This enables the optimization of production conditions and guides the selection of production cell lines. The manual workflow processes up to 192 samples per run and is completed in ~8–9 h; a detailed timeline is provided in Supplementary Table1.