Fig. 4: Application in cell line selection.
From: High-throughput glycosylation screening method for biologics development using MALDI-TOF-MS
Glycans 1–6: Man5, G0F-GN, G0, G0F, G1F and G0FB. a Example trend plot for Glycan 5 (G1F). MALDI intensities obtained with the +3 Da internal standard (left y-axis) parallel the UHPLC percentages (right y-axis) across all four lots. b Correlation between MALDI and UHPLC for the same glycan; least-squares fit gives R² = 0.99. Symbols denote individual lots. c Absolute MALDI read-outs (internal-standard normalisation) for Glycans 1–6 in the four lots. d UHPLC results (percentage values) for Glycans 1–6 in the four lots. e High-abundance glycans ( > 10% of the total pool). Triplicate measurements of G0F and G1F acquired with ( + IS, green circles) or without (–IS, orange triangles) internal standards. Data obtained with the +IS workflow are expressed as the signal-to-internal-standard ratio (S/I; left ordinate), whereas –IS data are plotted as relative intensity% (right ordinate). Braces indicate coefficients of variation (CV). f Low-abundance glycans ( < 10%). The same comparison for Man5, G0F-GN, and G0. The +IS method retains CVs ≤ 12.8%, while the variability of the –IS measurements rises markedly (CV = 21.8–41.6%). g Impact on sample discrimination. Relative levels of a representative low-abundance glycan in two process batches (S1 and S3), each analysed in triplicate. The tighter dispersion afforded by the +IS workflow allows the between-batch difference to be resolved, whereas the greater scatter in the –IS data obscures this distinction.
