Table 1 Repeatability and intermediate precision

From: High-throughput glycosylation screening method for biologics development using MALDI-TOF-MS

Glycan

Putative Structure

Composition

DAY1 (Mean ± SD)

Reproducibility CV

DAY2 (Mean ± SD)

DAY3 (Mean ± SD)

Cross Day CV

1

2

3

4

5

6

1

2

3

1

2

3

G0-GN

H3N3

0.320 ± 0.016

0.307 ± 0.006

0.328 ± 0.019

0.344 ± 0.007

0.273 ± 0.013

0.376 ± 0.010

10.76%

0.315 ± 0.018

0.352 ± 0.018

0.306 ± 0.031

0.340 ± 0.016

0.353 ± 0.004

0.383 ± 0.044

9.46%

Man5

H5N2

0.295 ± 0.005

0.281 ± 0.006

0.291 ± 0.008

0.295 ± 0.005

0.232 ± 0.003

0.348 ± 0.005

12.73%

0.255 ± 0.026

0.296 ± 0.013

0.238 ± 0.015

0.265 ± 0.006

0.280 ± 0.008

0.298 ± 0.033

11.08%

G0F-GN

H3N3F1

0.293 ± 0.008

0.252 ± 0.006

0.286 ± 0.010

0.291 ± 0.004

0.226 ± 0.004

0.314 ± 0.004

11.53%

0.227 ± 0.025

0.266 ± 0.013

0.214 ± 0.014

0.270 ± 0.003

0.285 ± 0.011

0.280 ± 0.030

11.61%

G0

H3N4

0.289 ± 0.008

0.278 ± 0.004

0.293 ± 0.010

0.294 ± 0.005

0.237 ± 0.006

0.344 ± 0.005

11.86%

0.261 ± 0.027

0.302 ± 0.021

0.246 ± 0.021

0.279 ± 0.008

0.294 ± 0.004

0.314 ± 0.035

10.18%

G0F

H3N4F1

0.587 ± 0.026

0.543 ± 0.024

0.552 ± 0.024

0.525 ± 0.034

0.485 ± 0.022

0.563 ± 0.022

6.44%

0.464 ± 0.035

0.483 ± 0.037

0.422 ± 0.030

0.470 ± 0.019

0.462 ± 0.020

0.439 ± 0.009

10.57%

G1F

H4N4F1

0.303 ± 0.005

0.269 ± 0.003

0.303 ± 0.011

0.311 ± 0.008

0.244 ± 0.006

0.341 ± 0.004

11.45%

0.248 ± 0.027

0.279 ± 0.017

0.233 ± 0.016

0.303 ± 0.001

0.313 ± 0.009

0.313 ± 0.034

11.55%

G0FB

H3N5F1

0.446 ± 0.013

0.428 ± 0.012

0.453 ± 0.014

0.496 ± 0.009

0.403 ± 0.004

0.479 ± 0.009

7.50%

0.438 ± 0.014

0.463 ± 0.062

0.442 ± 0.047

0.502 ± 0.017

0.542 ± 0.006

0.527 ± 0.036

8.93%

G2F

H5N4F1

0.303 ± 0.005

0.379 ± 0.016

0.416 ± 0.014

0.461 ± 0.033

0.350 ± 0.032

0.459 ± 0.043

11.02%

0.388 ± 0.036

0.428 ± 0.074

0.376 ± 0.062

0.477 ± 0.034

0.507 ± 0.011

0.517 ± 0.080

12.83%

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