Fig. 3: Comparison of the equilibrium size distribution of 30 μM JB6 at 22 °C (blue) and 37 °C (red).
From: Effects of solution conditions on the self-assembly of the chaperone protein DNAJB6b
a Mass photometry data (mass-weighted distributions) and a schematic illustration of the technique principle. Light is scattered more or less by single particles close to the surface, depending on their respective masses. b The same samples as in (b) analyzed with AUC in velocity sedimentation mode. Plotted are the temperature corrected sedimentation coefficients of 20 °C. The schematic illustrates how this technique relies on the detection of sedimentation velocity during centrifugation, depending on both the centrifugal force, FC, and the drag force, FD.
