Fig. 4: Micelle dissociation of JB6 at different temperatures.
From: Effects of solution conditions on the self-assembly of the chaperone protein DNAJB6b
a 〈RH〉, measured with microfluidic diffusional sizing, MDS, as a function of time since dilution, at temperatures (22−40 °C) as indicated by color and symbol in the legend. The error bars represent the standard deviation of three technical replicates. Each series was fitted to a single exponential decay function, \({\langle {{{{\rm{R}}}}}_{{{{\rm{H}}}}}\rangle }_{(t)}=a* {{{{\rm{e}}}}}^{-kt}+c\), shown as dashed lines. b Dissociation kinetics at 22 and 37 °C, probed by the change of total micelle mass during 60 s data collection with mass photometry. 34 μM non-labeled JB6 was diluted to 50 nM at time zero. c, d The change in mass distribution upon dilution at 22 respectively 37 °C. The distributions are plotted with shifted y-axis to allow for better comparison. e Arrhenius plot of the obtained dissociation rate constants (from MDS in black circles and from mass photometry in colored stars) versus the inverse of the temperature. The fitted line describes a thermally activated dissociation process, \({k}_{(T)}=A* {{{{\rm{e}}}}}^{-{E}_{a}/RT}\), where the activation energy, Ea, is found to be 250 kJ/mol.
