Fig. 5: pH dependence of the self-assembly of JB6.
From: Effects of solution conditions on the self-assembly of the chaperone protein DNAJB6b
a Light scattering intensity (here used as a turbidity reporter) of 10 μM JB6 as a function of pH. Titration with 20 mM phosphoric acid to a starting solution with pH 8.0, 20 mM NaP, 0.2 mM EDTA, at room temperature. b 〈RH〉 of 30 μM JB6 in 20 mM NaP at room temperature, measured with MDS at different pH values. Standard deviation of three technical replicates. The 〈RH〉 of a mainly monomeric mutant of JB6, denoted as ΔST, is shown in a gray pentagon as comparison. c Mass-weighted size distributions at pH 2-8, obtained with mass photometry of the same samples as in (b). Inset with average N of each mass distribution. d Distributions in sedimentation coefficients obtained by AUC of the same samples as in (b) together with the ΔST JB6 mutant. Each distribution is given by solutions to a Monte Carlo simulation that describes the noise reduced raw data absorbance scans, obtained in velocity sedimentation mode. The solutions are connected by a line to help distinguishing each pH series. The color and symbol representation in the legend are valid for panel (b−d).
