Fig. 2: Orthogroup analysis and selection of cytochrome P450 candidates for alkaloid scaffold oxidation.
From: Orthologue inference-based enzyme mining for diversification of the anti-cancer evodiamine scaffold
a OrthoFinder analysis identified orthologs of previously characterized alkaloid oxygenases. Bar charts show, for each species, the number of genes assigned to orthogroups (green) and the number of genes identified as orthologs (purple). Plant species from which the final 15 P450 candidates were selected for substrate screening are marked with red dots. Species highlighted in orange encode P450s with confirmed activity against alkaloid scaffolds, while those in blue provided the “bait” sequences. b Fifteen candidate P450s (red) were selected from the ortholog pool and arranged in a neighbor-joining tree with other P450s, including previously characterized alkaloid oxygenases (green) and other functionally characterized P450s (black). c The final set of 15 candidate P450s was manually curated from a pool of 547 orthogroup members.
