Fig. 7: Degree and positional effects of N-methylation on peptide cytosolic accumulation measured by CHAMP.

a Chemical structures of the N-methylation sub-library with varying degrees of backbone N-methylation (Nmet0-5). b Apparent accumulation of the N-methylation sub-library with varying degrees of backbone N-methylation using the CHAMP assay. HT HeLa cells treated with DBCOcl were pulsed with 50 μΜ of compounds for 24 h and chased with 50 μΜ of TMRaz. Data are represented as mean ± SD (n = 3). c Chemical structures of the N-methylation sub-library with varying positions of backbone N-methylation (Nmet6-9 and Nmet1). d Apparent accumulation of the N-methylation sub-library with varying positions of backbone N-methylation. HT HeLa cells treated with DBCOcl were pulsed with 50 μΜ of compounds for 24 h and chased with 50 μΜ of TMRaz. Data are represented as mean ± SD (n = 3). P-values were determined by a two-tailed t-test (* denotes a p-value  <  0.05, ** < 0.01, ***<0.001, ****<0.0001, ns = not significant).