Table 1 Mechanical characterization of C3 by single-molecule atomic force spectroscopy

From: Concurrent atomic force spectroscopy

AFM mode

Protein

C3, 〈Fu〉 (pN)

nexp

nevents

RSD at 3.6% CU (%)

RSD at 18% CU (%)

Traditional

(C3)8

90.7

11

1334

3.0

8.2

 

(C3-L)4

88.4

6

177

N/A

N/A

 

(C3-SUMO1)4

79.2

8

742

3.2

9.4

OFP, single marker

(C3)8

96.3

5

625

2.2

4.0

 

(C3-L)4

93.4

5

311

N/A

N/A

OFP, dual marker

(C3-L)4

90.0

14

873

N/A

N/A

 

(C3-SUMO1)4

89.5

14

1043

1.5

2.9

  1. The table summarizes results regarding mechanical characterization of the C3 domain in the context of three different polyproteins, following traditional atomic force microscopy (AFM) or orthogonal fingerpriting (OFP)-based concurrent measurements. nexp and nevents represent the number of experiments and unfolding events, respectively. Relative standard deviations (RSDs) of the distributions of \(\Delta \left\langle {F_{\mathrm{u}}} \right\rangle\) with respect to (C3-L)4 at two different calibration uncertainties (CU) were estimated by Monte Carlo simulations using the actual structure of the experimental datasets. N/A not applicable
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