Fig. 2
Control in time and space of the collective extrusion. a Appearance time Tap of the collective extrusion as a function of the region of illumination (ROI) diameter when the blue-pulse period Δt is set to 5 min (n = 10 samples from 1 experiment for each diameter). b Projected area Abud of the aggregate as a function of the area of the ROI AROI for Δt = 5 min (n = 10 samples from 1 experiment for each diameter). The underlying size of the aggregate is always larger than the ROI. The dashed line indicates y = x. c Appearance time Tap of the aggregate as a function of Δt for a ROI of diameter 70 μm. Tap is delayed as the period of blue-light pulses increases, suggesting that varying the amount of optoSrc at the membrane directly impacts the kinetics of the collective extrusion (n = 20 for Δt = 2 min; n = 15 for Δt = 5, 10, and 15 min; n = 10 for Δt = 20 min; n = 19 for Δt = 30 min and n = 10 for Δt = 40 min, all coming from at least 2 experiments). Horizontal pink lines correspond to mean values in (a–c). d Probability of observing a collective extrusion Pbud as a function of Δt for a ROI of diameter 70 μm: the slowed-down kinetics of the collective extrusion when the frequency of blue-light pulses decreases is accompanied with a decrease in collective extrusion events (n = 20 for Δt = 2 min; n = 15 for Δt = 5, 10, and 15 min; n = 10 for Δt = 20 min; n = 19 for Δt = 30 min and n = 10 for Δt = 40 min, all coming from at least 2 experiments). The error bars represent the uncertainty, as described in the Methods
