Fig. 5: Aspiration-assisted freeform bioprinting of circular cartilage tissues.
From: Aspiration-assisted freeform bioprinting of pre-fabricated tissue spheroids in a yield-stress gel
Strategy I: cartilages tissues were bioprinting using mesenchymal stem cell spheroids on Day 3 and removed from 0.5% alginate microparticles on Day 4. a A microscopic image showing a bioprinted construct after removal from alginate microparticles. b The final shape of the bioprinted cartilage at Day 24. Histological and immunostaining images of the bioprinted tissues at Day 24 including c hematoxylin and eosin (H&E), d toluidine blue, e Col-II, and f Aggrecan staining. Strategy II: cartilage tissues were bioprinted using chondrogenic spheroids at Day 22. g A microscopic image showing bioprinted construct after its removal from 0.5% alginate microparticles at Day 23. h The final shape of the bioprinted cartilage tissue at Day 24. Histological and immunostaining images of the bioprinted tissues at Day 24 including i H&E, j toluidine blue, k Col-II, and l Aggrecan. We showed that the bioprinted tissues in Strategy I exhibited chondrogenic properties; however, the bioprinted shape could not be retained because of the compaction of MSC spheroids. In Strategy II, we observed sufficient fusion between spheroids and the originally bioprinted circular arrangement was retained. The red dashed line denotes the region magnified.
