Fig. 3: Membrane order changes in SLBs induced by LecA. | Communications Physics

Fig. 3: Membrane order changes in SLBs induced by LecA.

From: The bacterial lectin LecA from P. aeruginosa alters membrane organization by dispersing ordered domains

Fig. 3

Two-color imaging was performed using confocal microscopy. a GP images of the phase-separated SLB (DOPC/chol/SM/Gb3 (37.5/20/37.5/5)) before and at several time points after LecA application. The color bar on the right panel encodes the GP values. Phase dispersion (pink arrowhead) occured in the same way as in Fig. 1. The membrane multilayers are highlighted by ROIs with white borderlines. b GP histograms of the SLB at 0 min (black curve), 10 min (red curve), and 18 min (blue curve) after LecA application. c Membrane order of Lo domains and Ld domains at 0 at 18 min and membrane multilayers at 18 min after LecA addition. Every pixel within the ROI of Ld, Lo, or multilayers is a separate data point here, the number of processed pixels is displayed in brackets. Scale bars are 10 and 5 µm, respectively. The ratiometric imaging is available in Fig. S5 and Supplementary Movie 2. All quantifications are provided for the single image displayed in (a).

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