Extended Data Fig. 4: Images acquired by the RL agent in a real experiment on a different microscope.
From: Development of AI-assisted microscopy frameworks through realistic simulation with pySTED
NPC was stained with the STAR-RED fluorophore. The sequence of acquired images goes from top left to bottom right. The confocal images before (CONF1) and after (CONF2) are presented for photobleaching comparison. The CONF1 image is normalized to the CONF2 image. The STED images are normalized to the 99th percentile of the intensity of the CONF1 image. Images are 5.12μm × 5.12μm. The evolution of the parameter selection (left; STED: STED power, Exc.: Excitation power, Pdt.: Pixel dwelltime) and imaging optimization objectives (right; R: Resolution, P: Photobleaching, S: Signal ratio) are presented.
